創世紀代理產品討論區
| A1: | Immunochemistry kit常見問題，請參閱。 | ||
| A2: | Mirus labeling kit常見問題，請參閱。 | ||
| A3: | 1. | 不具侵略性: 一般reagent以liposome為媒介方式,,但liposome在與bi-layer membrane 融合時,較易將包外有毒物質一併帶入.對細胞造成傷害 | |
| 2. | 低毒性:reagent 本身對細胞的傷害 | ||
| 3. | Off-target effects:越多的siRNA conc.越有機會造成non-specific effect! | ||
| 4. | Antibiotic and serum compatible: medium 內含有antibiotic 可避免transfection 過程中汙染的機會; medium 內含有serium, 不會中止細胞在transfect 期間的生長. | ||
| 5. | 低cell confluence: 低的細胞量,其transfect效率越高,並且,當基因incubate 越久才會表現時,越需更少量的細胞量,才不會在48,72hr時cell 過量,造成細胞狀況不好 | ||
| 6. | 高的擴散率: siRNA + reagent complex 進入cell後,越早均勻擴散.實驗成功機會越高 | ||
| A4: | 可以, Polyplus於2008年8月推出jetPEI新的protocol,讓您操作上更方便 簡單,所以新的protocol是可以試用在之前賣出和現在的jetPEI,新的protocol 請見附件! |
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| A5: | 您好,建議使用INTERFERin來送siRNA進入Cortical neuron 以下是原廠測試的操作方法: |
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| - Seed 50 000 cells per well in precoated plates (polylysine) | |||
| - Transfect the cells 4 days after seeding them | |||
| - Test 20 and 50 nM siRNA | |||
| - Use 2 µl of INTERFERin per well | |||
| - Before transfection, remove cell culture medium and store the
medium at 37°C. |
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| - During transfection, maintain the cells in 1 ml of neurobasal
medium for 4 hours |
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| - 4 hours after transfection, replace transfection medium by 50% old
medium 50% fresh complete medium |
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